The curious collection of a slightly mad scientist
The detailed changes in the structure of a virus as it infects an E. coli bacterium have been observed for the first time, report researchers from The University of Texas at Austin and The University of Texas Health Science Center at Houston (UT Health) Medical School this week in Science Express.
To infect a cell, a virus must be able to first find a suitable cell and then eject its genetic material into its host. This robot-like process has been observed in a virus called T7 and visualized by Ian Molineux, professor of biology at The University of Texas at Austin, and his colleagues.
The researchers show that when searching for its prey, the virus briefly extends — like feelers — one or two of six ultra-thin fibers it normally keeps folded at the base of its head.
Once a suitable host has been located, the virus behaves a bit like a planetary rover, extending these fibers to walk randomly across the surface of the cell and find an optimal site for infection.
At the preferred infection site, the virus goes through a major change in structure in which it ejects some of its proteins through the bacterium’s cell membrane, creating a path for the virus’s genetic material to enter the host.
After the viral DNA has been ejected, the protein path collapses and the infected cell membrane reseals.
“Although many of these details are specific to T7,” said Molineux, “the overall process completely changes our understanding of how a virus infects a cell.”
For example, the researchers now know that most of the fibers are usually bound to the virus head rather than extended, as was previously thought. That those fibers are in a dynamic equilibrium between bound and extended states is also new.
Molineux said that the idea that phages “walk” over the cell surface was previously proposed, but their paper provides the first experimental evidence that this is the case. …